We present here a protocol on how to prepare primary cultures of glial cells, astrocytes, and microglia from rat cortices for time-lapse video imaging of intracellular Ca²⁺ for research on pathophysiology of amyotrophic lateral sclerosis in the hSOD1^G93A rat model.

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Milićević, K., Korenić, A., Milošević, M., Andjus, P. R. Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis. J. Vis. Exp. (184), e63483, doi:10.3791/63483 (2022).